Title of the article

A differentiated Ca2+ signalling phenotype has minimal impact on myocardin expression in an automated differentiation assay using A7r5 cells.

List of authors

BaRun Kim, Renato Molina, Gaby Jensen, Damon Poburko

The journal in which the article is published

Cell Calcium
doi: 10.1016/j.ceca.2021.102369. Online ahead of print.PMID: 33677175

 A brief summary

Vascular smooth muscle takes on at least two forms, a mature contractile form and a proliferating, migratory form that is linked to development and disease. Shifting from proliferative to contractile form is associated with increased expression of the transcription factor myocardin and changes in Ca2+ signalling. To test if the Ca2+ signalling impacts the myocardin expression, we imaged Ca2+signals in live cells then imaged myocardin and contractile proteins in those cells. This let is show that changes in Ca2+ signalling as the cells mature have little impact on myocardin expression, which will help us better understand vascular disease progression.